Presented at the Twentieth Annual Midwinter Research Meeting of the Association for Research in Otolaryngology, St. Petersburg, Florida, February 2–6, 1997.
Identification of MUC5B Mucin Gene in Human Middle Ear With Chronic Otitis Media†
Article first published online: 14 MAY 2009
Copyright © 2000 The Triological Society
Volume 110, Issue 4, pages 668–673, April 2000
How to Cite
Kawano, H., Paparella, M. M., Ho, S. B., Schachern, P. A., Morizono, N., Le, C. T. and Lin, J. (2000), Identification of MUC5B Mucin Gene in Human Middle Ear With Chronic Otitis Media. The Laryngoscope, 110: 668–673. doi: 10.1097/00005537-200004000-00024
Supported in part by Multiple District (5M) Lion's Hearing Foundation, the International Hearing Foundation, and National Institutes of Health Grant No. 5P50 DC03093 from the National Institute on Deafness and Other Communication Disorders.
- Issue published online: 14 MAY 2009
- Article first published online: 14 MAY 2009
- Manuscript Accepted: 7 JAN 2000
Objectives To identify the mucin gene and its expressing cells in the middle ear mucosa with chronic otitis media (COM), and to study the correlation between infiltration of inflammatory cells in the submucosa and expression of the mucin gene in the mucosal epithelium with COM.
Study Design Middle ear mucosal specimens removed from the inferior promontory area of 19 patients undergoing middle ear surgery for COM were studied.
Methods Sections were stained with H&E, Alcian blue-periodic acid Schiff (AB-PAS), polyclonal MUC5B antibody, and specific MUC5B riboprobe for histological, histochemical, immunohistochemical, and mucin mRNA analyses.
Results H&E staining revealed pseudostratified epithelia in 18 of the middle ear specimens with COM and cuboidal secretory epithelia in one. AB-PAS staining of epithelia revealed abundant secretory cells and their products (glycoconjugates). In situ hybridization and immunohistochemistry studies demonstrated that the secretory cells of the middle ear mucosa with COM expressed MUC5B mucin mRNA and its product MUC5B mucin.
Conclusions The MUC5B mucin gene and its product were identified in the middle ear secretory cells of patients with COM. Its e-pression was e-tensive in pseudostratified mucosal epithelia and related to infiltration of inflammatory cells in the submucosa of the middle ear cleft with COM, suggestive that inflammatory cell products are involved in the production of MUC5B.