Article

Inhibition of the c-Jun N-Terminal Kinase Signaling Pathway Influences Neurite Outgrowth of Spiral Ganglion Neurons In Vitro

  1. Daniel Bodmer MD, PhD1,
  2. Bertrand Gloddek MD1,
  3. Allen F. Ryan PhD1,*,
  4. Jochen Huverstuhl MD1,
  5. Dominik Brors MD2

Article first published online: 2 JAN 2009

DOI: 10.1097/00005537-200211000-00028

Issue

The Laryngoscope

The Laryngoscope

Volume 112, Issue 11, pages 2057–2061, November 2002

Additional Information

How to Cite

Bodmer, D., Gloddek, B., Ryan, A. F., Huverstuhl, J. and Brors, D. (2002), Inhibition of the c-Jun N-Terminal Kinase Signaling Pathway Influences Neurite Outgrowth of Spiral Ganglion Neurons In Vitro. The Laryngoscope, 112: 2057–2061. doi: 10.1097/00005537-200211000-00028

Author Information

  1. 1

    Division Otolaryngology, Department of Surgery, University of California San Diego School of Medicine, and Veterans Affairs Medical Center, La Jolla, California, U.S.A.

  2. 2

    Clinic for Otolaryngology—Head and Neck Surgery, University Hospital Zurich, Zurich, Switzerland

*University of California San Diego School of Medicine, 9500 Gilman Drive, 0666, La Jolla, CA 92093, U.S.A.

Publication History

  1. Issue published online: 2 JAN 2009
  2. Article first published online: 2 JAN 2009
  3. Manuscript Accepted: 22 MAY 2002

Funded by

  • NIH, NIDCD, and the VA Medical Center

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Keywords:

  • c-Jun N-terminal kinase;
  • neurite;
  • rat;
  • spiral ganglion neurons

Abstract

Objectives Inhibitors of the c-Jun N-terminal kinase (JNK) signaling pathway have been demonstrated to protect hair cells of the auditory system and different types of neurons from various insults, and their use for future therapeutic applications has been proposed. In the study, we evaluated the effects of inhibition of the JNK pathway on process outgrowth from spiral ganglion neurons.

Methods Spiral ganglion explants from rats (postnatal days 3–5) that were cultured on laminin were treated with neurotrophin-3 and/or the JNK signaling pathway inhibitor CEP-11004. Both neurite length and number of the explants were evaluated and statistically analyzed by analysis of variance.

Results Inhibition of the JNK signaling pathway reduced process outgrowth from spiral ganglion explants. The reduction, both in length and number of neurites, was reversed by the application of neurotrophin-3.

Conclusions The results indicate that an intact JNK signaling pathway is important for process outgrowth of spiral ganglion neurons. However, neurotrophin-3 stimulates process extension by a JNK independent pathway. Our results demonstrate that inhibition of the JNK pathway can have adverse effects on the extension of spiral ganglion neurons, but that the negative effects can be ameliorated by appropriate treatment.

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