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Keywords:

  • Cholesteatoma;
  • complementary DNA array;
  • reverse transcriptase-polymerase chain reaction;
  • calgranulins;
  • cathepsins

Abstract

Objective To identify genes regulated in human cholesteatoma compared with normal skin tissue using complementary DNA arrays.

Study Design In vitro analysis.

Methods Eight cholesteatoma and retroauricular skin samples were obtained from the same patients during surgery. Upregulated and downregulated genes were highlighted using complementary DNA arrays for screening. Reverse transcriptase-polymerase chain reaction and immunohistochemical staining were performed to confirm the results of the complementary DNA array.

Results Twelve genes were found to be induced or upregulated in cholesteatoma compared with skin samples. These included genes involved in cell proliferation and differentiation (eg, calgranulin A, calgranulin B, psoriasin, thymosin β-10) and cell invasion (eg, cathepsin C, cathepsin D, cathepsin H). Analyses by means of reverse transcription-polymerase chain reaction showed enhanced expression of several genes including calgranulin A, calgranulin B, psoriasin, thymosin β-10, cathepsin C, cathepsin D, and cathepsin H in cholesteatoma, supporting the findings from the gene array. In addition, it was verified by immunohistochemical analysis that the expressions of Calgranulin A, Calgranulin B, and Cathepsin D were mainly located in cholesteatoma epithelium.

Conclusion The observed alteration in gene expression may play a role in various mechanisms of pathogenesis in cholesteatoma.