Presented as a preliminary report at the 5th Extraordinary International Symposium on Recent Advances in Otitis Media, Sendai, Japan, April 17–20, 2001.
Article first published online: 9 SEP 2010
Copyright © 2003 The Triological Society
Volume 113, Issue 11, pages 2047–2051, November 2003
How to Cite
Eriksson, P. O. and Hellström, S. (2003), Acute otitis media develops in the rat after intranasal challenge of Streptococcus pneumoniae. The Laryngoscope, 113: 2047–2051. doi: 10.1097/00005537-200311000-00036
Supported by Swedish Medical Research Council grant K2001−73X-06578-19A, the Medical Faculty, Umeå University, and Spjutspetsmedel from the County Council of Vasterbotten.
- Issue published online: 9 SEP 2010
- Article first published online: 9 SEP 2010
- Manuscript Accepted: 19 MAY 2003
- Acute otitis media;
- Streptococcus pneumoniae;
- eustachian tube;
- compound 48/80
Objectives/Hypothesis: The rat is a frequently used animal model for middle ear research. To date, acute otitis media (AOM) has been evoked after instillation of bacteria directly into the middle ear cavity or after traumatizing the tympanic membrane. The purpose of the study was to examine whether, with an intact tympanic membrane and middle ear cavity, intranasally deposited bacteria cause AOM and how tympanic membrane stimulation influences this procedure. Study Design: In vivo, murine model. Methods: In a rat model, Streptococcus pneumoniae, type 3, was intranasally inoculated for 5 consecutive days. The tympanic membrane was treated with saline or with compound 48/80 or was left untreated. The development of AOM was evaluated by otomicroscopy, light microscopy, and middle ear culture. Results: Ninety percent of the ears developed AOM. However, when the tympanic membranes were treated with saline or compound 48/80, only 40% and 57%, respectively, developed AOM. In all, 23 of 40 ears developed AOM and 20 ears showed growth of bacteria. Conclusion: Repeated intranasal deposition of S pneumoniae, type 3, causes AOM in the rat. The development of AOM can be influenced by tympanic membrane stimulation.