The expression of IL-12 p40 and its homologue, epstein-barr virus-induced gene 3, in inflammatory bowel disease
Article first published online: 14 DEC 2006
Copyright © 2001 Crohn's & Colitis Foundation of America, Inc.
Inflammatory Bowel Diseases
Volume 7, Issue 3, pages 215–220, August 2001
How to Cite
Omata, F., Birkenbach, M., Matsuzaki, S., Christ, A. D. and Blumberg, R. S. (2001), The expression of IL-12 p40 and its homologue, epstein-barr virus-induced gene 3, in inflammatory bowel disease. Inflamm Bowel Dis, 7: 215–220. doi: 10.1097/00054725-200108000-00006
- Issue published online: 14 DEC 2006
- Article first published online: 14 DEC 2006
- Manuscript Accepted: 28 MAR 2001
- Manuscript Received: 11 OCT 2000
- Epstein-Barr virus-induced gene 3;
- Ulcerative colitis;
- Crohn's disease;
- Real-time polymerase chain reaction;
It has recently been suggested that Crohn's Disease (CD) is associated with an exaggerated T helper 1 cytokine response as manifest by increased production of interleukin-12 (IL-12) and interferon-γ (IFN-γ). Epstein-Barr virus-induced gene 3 (EBI3) encodes a 34-kDa glycoprotein that is 27% identical to the p40 unit of IL-12 and has recently been reported to be up-regulated in ulcerative colitis (UC). Aim To determine whether mucosal expression of IL-12 p40 or EBI3 correlates with inflammatory bowel disease (IBD). Patients/Methods mRNA expression in colonic mucosa from patients with UC, Crohn's disease (CD) and non-IBD controls was measured by reverse-transcribed real-time polymerase chain reaction (PCR).
EBI3 was significantly increased in both involved and uninvolved colonic mucosa in patients with UC. Although IL-12 p40 was increased in some patients with CD relative to non-IBD controls, the increase was not statistically significant. However, 5-aminosalicylic acid (5-ASA) use was significantly correlated with reduced IL-12 p40 levels in the patients with CD, but not in UC cases. A similar reduction was not seen in 5-ASA-treated UC patients.
IL-12 p40 expression in CD is heterogeneous. In contrast, expression of the IL-12 p40 homologue, EBI3, is up-regulated in nearly all UC cases and in a subset of CD.