Prevention of fibrosis in experimental colitis by captopril: The role of tgf-β1
Version of Record online: 14 DEC 2006
Copyright © 2004 Crohn's & Colitis Foundation of America, Inc.
Inflammatory Bowel Diseases
Volume 10, Issue 5, pages 536–545, September 2004
How to Cite
Wengrower, D., Zannineli, G., Pappo, O., Latella, G., Sestieri, M., Villanova, A., Faitelson, Y., Pines, M. and Goldin, E. (2004), Prevention of fibrosis in experimental colitis by captopril: The role of tgf-β1. Inflamm Bowel Dis, 10: 536–545. doi: 10.1097/00054725-200409000-00007
- Issue online: 14 DEC 2006
- Version of Record online: 14 DEC 2006
- Manuscript Accepted: 17 OCT 2003
- Manuscript Received: 11 OCT 2003
- experimental colitis;
- Crohn's disease;
Background & Aims:
There is a body of evidence to suggest that the local activation of angiotensin II (ANG II) plays a pivotal role in fibrogenic response involving the kidney, heart, lung, pancreas and liver. In such conditions, fibrosis is mediated, at least partially, through ANG II induction of the cytokine transforming growth factor-beta1 (TGF-β1). Both ANG II and TGF-β1 also seem to be involved in intestinal fibrosis and stenosis, particularly in Crohn's disease.
The aim of the present study was, firstly, to determine the effects of the angiotensin-converting enzyme inhibitor, captopril, on colonic fibrosis in experimental colitis in rats and, secondly, to check the role of TGF-β1 on these effects.
Colitis was induced in rats by intracolonic administration of TNBS. Colonic fibrosis was assessed 21 days later by macroscopic and microscopic evaluation. Levels of collagen α1 gene expression, hydroxyproline, angiotensin II and TGF-β1 proteins, and TGF-β1 mRNA were measured on the colonic tissue. Results: In chronic colitis, captopril significantly reduced the score of macroscopic and histologic lesions, as well as the colonic tissue levels of collagen α1, hydroxyproline, ANG II and TGF-β1 proteins, and TGF-β1 mRNA.
These data demonstrate for the first time that the prophylactic administration of captopril is effective in preventing colonic fibrosis in TNBS-induced colitis. The antifibrotic action of captopril could be due to the blockade of TGFβ-1 overexpression, and/or to a direct down-regulation of TGFβ-1 transcript.