Dose-Dependent Activation of Ca2+-Activated K+ Channels by Ethanol Contributes to Improved Endothelial Cell Functions
Article first published online: 3 MAY 2006
Alcoholism: Clinical and Experimental Research
Volume 28, Issue 7, pages 1005–1011, July 2004
How to Cite
Kuhlmann, C. R. W., Li, F., Lüdders, D. W., Schaefer, C. A., Most, A. K., Backenköhler, U., Neumann, T., Tillmanns, H., Waldecker, B., Erdogan, A. and Wiecha, J. (2004), Dose-Dependent Activation of Ca2+-Activated K+ Channels by Ethanol Contributes to Improved Endothelial Cell Functions. Alcoholism: Clinical and Experimental Research, 28: 1005–1011. doi: 10.1097/01.ALC.0000130811.92457.0D
- Issue published online: 3 MAY 2006
- Article first published online: 3 MAY 2006
- Received for publication January 21, 2004; accepted April 14, 2004.
- Patch-Clamp Technique;
- Endothelial Dysfunction
Background: Regular moderate alcohol (EtOH) intake seems to protect against both coronary artery disease and ischemic stroke, whereas the risk increases with heavy EtOH consumption. Effects of EtOH on endothelial cell function may be relevant to these disparate effects. Potassium channels play an important role in the regulation of endothelial cell functions. Therefore, we investigated whether Ca2+-activated K+ channels (BKCa) are modulated by EtOH. Furthermore, we examined whether EtOH-induced changes of endothelial nitric oxide (NO) formation and cell proliferation are due to BKCa activation.
Methods: The patch-clamp technique was used to investigate BKCa activity in cultured human umbilical vein endothelial cells (HUVEC). NO formation was analyzed by using the fluorescence dye 4,5-diaminofluorescein. Endothelial proliferation was examined by using cell counts and measuring [3H]thymidine incorporation.
Results: EtOH dose-dependently (10–150 mmol/liter) modulated BKCa-activity, with the highest increase of open-state probability at a concentration of 50 mmol/liter (n= 13; p < 0.05). Inside-out recordings revealed that this effect was due to direct BKCa activation, whereas open-state probability was not changed in cell-attached recordings after pertussis toxin preincubation. EtOH (10 and 50 mmol/liter) caused a significant increase of NO levels, which was blocked by the highly selective BKCa inhibitor iberiotoxin (100 nmol/l; n= 30; p < 0.05). Higher concentrations of EtOH (100 and 150 mmol/liter) significantly reduced NO synthesis (n= 30; p < 0.05). Both methods revealed a significant increase of HUVEC proliferation, which was inhibited by iberiotoxin (n= 30; p < 0.05). At a concentration of 150 mmol/liter, EtOH caused a significant reduction of endothelial proliferation.
Conclusions: EtOH directly activates BKCa in HUVEC, leading to an increase of endothelial proliferation and production of NO. These results indicate a possible beneficial effect of low-dose EtOH on endothelial function, whereas higher concentrations must be considered as harmful.