Effects of Systemic and Local CXC Chemokine Administration on the Ethanol-Induced Suppression of Pulmonary Neutrophil Recruitment

Authors

  • Lee J. Quinton,

    1. Department of Physiology (LJQ, SN, GJB), the Department of Medicine, Section of Pulmonary Critical Care Medicine (SN, PZ, KIH, LG), and the Alcohol Research Center (LJQ, SN, PZ, KIH, LG, GJB), Louisiana State University Health Sciences Center, New Orleans, Louisiana.
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  • Steve Nelson,

    1. Department of Physiology (LJQ, SN, GJB), the Department of Medicine, Section of Pulmonary Critical Care Medicine (SN, PZ, KIH, LG), and the Alcohol Research Center (LJQ, SN, PZ, KIH, LG, GJB), Louisiana State University Health Sciences Center, New Orleans, Louisiana.
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  • Ping Zhang,

    1. Department of Physiology (LJQ, SN, GJB), the Department of Medicine, Section of Pulmonary Critical Care Medicine (SN, PZ, KIH, LG), and the Alcohol Research Center (LJQ, SN, PZ, KIH, LG, GJB), Louisiana State University Health Sciences Center, New Orleans, Louisiana.
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  • Kyle I. Happel,

    1. Department of Physiology (LJQ, SN, GJB), the Department of Medicine, Section of Pulmonary Critical Care Medicine (SN, PZ, KIH, LG), and the Alcohol Research Center (LJQ, SN, PZ, KIH, LG, GJB), Louisiana State University Health Sciences Center, New Orleans, Louisiana.
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  • Lisa Gamble,

    1. Department of Physiology (LJQ, SN, GJB), the Department of Medicine, Section of Pulmonary Critical Care Medicine (SN, PZ, KIH, LG), and the Alcohol Research Center (LJQ, SN, PZ, KIH, LG, GJB), Louisiana State University Health Sciences Center, New Orleans, Louisiana.
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  • Gregory J. Bagby

    Corresponding author
    1. Department of Physiology (LJQ, SN, GJB), the Department of Medicine, Section of Pulmonary Critical Care Medicine (SN, PZ, KIH, LG), and the Alcohol Research Center (LJQ, SN, PZ, KIH, LG, GJB), Louisiana State University Health Sciences Center, New Orleans, Louisiana.
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  • This work was supported by grants AA07577 and AA09803 from the National Institute of Health.

Reprint requests: Gregory J. Bagby, PhD, Department of Physiology, Louisiana State University Health Sciences Center, 1901 Perdido St., New Orleans, LA 70112; Fax: 504-568-6158; E-mail, gbagby@lsuhsc.edu

Abstract

Background:

Acute alcohol intoxication impairs the neutrophil response to intrapulmonary infection, resulting in impaired host defense and increased patient morbidity and mortality. We recently showed that intratracheal (IT) chemokine administration promotes pulmonary neutrophil migration in rats and that this process is enhanced by systemic administration of the Glu-Leu-Arg (ELR+) and CXC chemokine cytokine-induced neutrophil chemoattractant (CINC). Here we hypothesized that exogenous chemokine administration would mitigate the suppressive effect of alcohol on neutrophil recruitment into the lung.

Methods:

Macrophage inflammatory protein-2 (MIP-2), a rat ELR+ CXC chemokine, or live Klebsiella pneumoniae (K. pneumoniae) was administered it to induce alveolar neutrophil migration in the absence or presence of acute ethanol intoxication. Depending on the experimental protocol, rats received either intravenous (IV) CINC or IT chemokines (CINC and MIP-2) 20 min after it MIP-2 or K. pneumoniae. Rats were euthanized 90 min or four hr after the first IT injection for sample collection.

Results:

Neutrophil counts were significantly elevated in bronchoalveolar lavage fluid (BALF) of rats receiving IT MIP-2 compared with vehicle-treated rats, and this response was significantly decreased in animals pretreated with ethanol. CINC IV enhanced the neutrophil response to IT MIP-2 in both the absence and presence of acute ethanol intoxication. In rats challenged with K. pneumoniae, ethanol pretreatment significantly reduced BALF levels of CINC and MIP-2, suppressed alveolar neutrophil recruitment, and decreased whole-lung myeloperoxidase activity. CINC IV did not alter BALF neutrophil counts in the absence or presence of ethanol administration 4 hr after IT K. pneumoniae. Alternatively, IT chemokine instillation partially restored BALF neutrophil recruitment but not whole-lung myeloperoxidase activity in ethanol-treated rats.

Conclusions:

Ethanol significantly inhibits the pulmonary inflammatory responses to both MIP-2 and K. pneumoniae. Exogenous chemokine administration may be a useful means to enhance host defenses in the ethanol-intoxicated host, although the results of this study also indicate that ethanol intoxication can impair neutrophil recruitment, independent of its effects on local chemotactic gradients.

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