Emma Barker was funded by the MRC, Mick Bailey by the BBSRC, and Martin Birchall by the Wellcome Trust.
An Ex Vivo Model for Reperfusion of Laryngotracheal Grafts†
Article first published online: 3 JAN 2009
Copyright © 2005 The Triological Society
Volume 115, Issue 4, pages 699–702, April 2005
How to Cite
Barker, E., Macchiarini, P., Murison, P., Jones, A., Haverson, K., Bailey, M. and Birchall, M. (2005), An Ex Vivo Model for Reperfusion of Laryngotracheal Grafts. The Laryngoscope, 115: 699–702. doi: 10.1097/01.mlg.0000161330.75600.4b
This work was undertaken in the Department of Clinical Veterinary Sciences, University of Bristol, Bristol, UK.
- Issue published online: 3 JAN 2009
- Article first published online: 3 JAN 2009
- Manuscript Accepted: 14 DEC 2004
Objective: To describe the development of an ex vivo model to facilitate the study of ischemia-reperfusion injury in laryngotracheal grafts taken from Minnesota minipigs.
Study Design: This is a descriptive study.
Methods: Laryngotracheal grafts from Minnesota minipigs were harvested and placed in cold storage for 3 hours. Autologous blood was used to reperfuse the graft for up to 8 hours using the described reperfusion model.
Results: By altering retrieval technique and pressures within the graft, we demonstrated healthy grafts after 8 hours of reperfusion.
Conclusions: Ex vivo reperfusion offers a range of experimental advantages over in vivo reperfusion including close control of interventions, ease of outcome measurement, and reduction in animal use. This model is now ready to be used for further studies of interventions to reduce ischemia-reperfusion injury in these grafts.