This work was generously supported by grants from Hungarian Scientific Research Fund (OTKA K63743, OTKA F035251, ETT 010/2001, and ETT 415/2003).
Detection of Osteoprotegerin and TNF-alpha mRNA in Ankylotic Stapes Footplates in Connection With Measles Virus Positivity†
Version of Record online: 2 JAN 2009
Copyright © 2006 The Triological Society
Volume 116, Issue 8, pages 1427–1433, August 2006
How to Cite
Karosi, T., Jókay, I., Kónya, J., Szabó, L. Z., Pytel, J., Jóri, J., Szalmás, A. and Sziklai, I. (2006), Detection of Osteoprotegerin and TNF-alpha mRNA in Ankylotic Stapes Footplates in Connection With Measles Virus Positivity. The Laryngoscope, 116: 1427–1433. doi: 10.1097/01.mlg.0000225928.35838.e5
- Issue online: 2 JAN 2009
- Version of Record online: 2 JAN 2009
- Manuscript Accepted: 28 FEB 2006
- Measles virus;
- stapes fixation;
Hypothesis: Otosclerosis is a bone remodeling disorder of the otic capsule causing conductive and sensorineural hearing loss. Persistent measles virus infection of the temporal bone with increased tumor necrosis factor (TNF)-alpha and decreased osteoprotegerin mRNA expression is supposed to be the main etiologic factor in otosclerosis.
Background: Determinants of measles virus infection and reactive inflammation were studied in otosclerosis. The presence of measles virus was shown in otosclerotic patients by reverse transcriptase-polymerase chain reaction (RT-PCR) amplification of the viral RNA. No report is available, however, about the role and interactions of bone-specific cytokines in otosclerosis.
Methods: Nucleic acid was extracted from stapes footplates of clinically otosclerotic patients. Measles virus nucleoprotein RNA was amplified by seminested RT-PCR. TNF-alpha and osteoprotegerin mRNA coexpression was detected by RT-PCR in otosclerotic bone and was correlated to measles virus positivity.
Results: Among 154 clinically stapes fixation otosclerotic patients, 99 stapedes contained measles virus RNA. TNF-alpha mRNA was detectable in 88 virus-positive and in 6 virus-negative stapes footplates. Osteoprotegerin mRNA expression was significantly lower in the TNF-alpha-positive specimens (P < .0001) that was independent from virus positivity.
Conclusion: Detection of TNF-alpha mRNA demonstrates activated osteoclast functions and inflammatory pathways in otosclerotic stapes footplates associated with measles virus presence. Increased expression of TNF-alpha and its action on RANK production inhibits the protective functions of osteoprotegerin on normal bone turnover in the otic capsule.