Activated Osteoclasts with CD51/61 Expression in Otosclerosis
Version of Record online: 2 JAN 2009
Copyright © 2006 The Triological Society
Volume 116, Issue 8, pages 1478–1484, August 2006
How to Cite
Karosi, T., Jókay, I., Kónya, J., Petkó, M., Szabó, L. Z., Pytel, J., Jóri, J. and Sziklai, I. (2006), Activated Osteoclasts with CD51/61 Expression in Otosclerosis. The Laryngoscope, 116: 1478–1484. doi: 10.1097/01.mlg.0000227251.67251.1f
- Issue online: 2 JAN 2009
- Version of Record online: 2 JAN 2009
- Manuscript Accepted: 27 MAR 2006
- Alcalic phosphatase;
- measles virus;
Hypothesis: Stapes ankylosis is supposed to be a disease with variable histopathology caused by otosclerosis or pseudo-otosclerosis. Persistent measles virus infection of the otic capsule could induce reactivation of quiescent embryonic osteoclasts in otosclerosis.
Background: Presence of measles virus RNA was demonstrated in the footplates of otosclerotic patients by reverse-transcription polymerase chain reaction (RT-PCR). Histology of active otosclerosis is featured by the presence of numerous osteoclasts with unknown phenotype.
Methods: Nucleic acid was extracted from stapes footplates of clinically otosclerotic patients (n = 261). Genomic RNA of measles virus was amplified by RT-PCR. Amplification results were correlated to postoperative histologic and CD51/61 specific immunohistologic findings. A parallel alcalic phosphatase activity assessment was performed to evaluate the metabolic activity of osteoclasts in each section.
Results: Among 261 stapes fixation cases, 175 otosclerotic stapes contained measles virus RNA. Histology for virus negative stapes (n = 86) represented nonotosclerotic, degenerative disorders. Histologically confirmed otosclerosis was featured by the presence of osteoclasts with renewed, embryonic phenotype. In otosclerosis, alcalic phosphatase activity was significantly higher compared with nonotosclerotic stapes ankylosis (P < .001).
Conclusion: The presence of CD51/61 positive osteoclasts in otosclerotic bone containing viral sequences provides the basis for an inflammatory bone remodeling disorder. Otosclerosis is a disease caused by persistent measles virus infection and reactivation of resting embryonic osteoclasts in the otic capsule.