Objectives: To analyze the presence and distribution of collagen fibers and versican in human vocal fold lamina propria of fetal larynges.
Study Design: Cross sectional analysis of cadaveric vocal folds of human fetuses.
Methods: Seven fetal larynges obtained from 28- to 36-week-old fetuses were analyzed with the Picrosirius-polarization method, immunohistochemistry, and image analysis.
Results: Collagen fibers within the lamina propria exhibited a monolaminar distribution pattern and spatial arrangement in “wicker basket.” Versican distribution was larger in the superficial and intermediate layers when compared to the deep layer.
Conclusion: Our findings suggest that collagen and versican distribution and arrangement within the lamina propria in the developing fetus are important for vocalization at birth.