• Chronic hyperplastic sinusitis;
  • fibroblasts;
  • cytokine;
  • nasal polyps;
  • TGFâ


Objectives: Transforming growth factor β-1 (TGF-β1) is a known fibrogenic factor with immunosuppressive properties. We wanted to determine the effect of stimulation with TGF-β1 on nasal polyp-derived fibroblasts and assess the role this molecule would have in polyp formation and growth.

Study Design: Nasal-polyp derived fibroblasts were cultured with or without TGF-β1, and proliferation and cytokine secretion were measured.

Methods: Fibroblasts were isolated from nasal polyps following endoscopic surgery. Cells were plated and grown until confluent, after which they were split and used in assays. Cells were stimulated with TGF- β1 and mRNA collected after 16 hours, supernatants after 72 hours, and proliferation measured after 96 hours of culture.

Results: TGF-β1 significantly (P < .02) increased proliferation of nasal-polyp derived fibroblasts. We examined the expression of inflammatory cytokines and found that TGF-β1 decreased expression of CCL2 (MCP-1), CCL5 (RANTES), CCL11 (eotaxin), granulocyte-colony stimulating factor (G-CSF), and GM-CSF (P < .05). In contrast, incubation with TGF-β1 increased fibronectin, procollagen, vascular endothelial growth factor (VEGF), and TGF-β2 protein production (P < .05). For select samples, we confirmed that the increased protein production was due to increased mRNA expression.

Conclusion: These studies suggest that TGF-β1 expression in polyp tissue can have dual effects. One role is to act as an anti-inflammatory agent shown by the ability to inhibit pro-inflammatory mRNA and protein production. At the same time, TGF-β1 expression leads to increases in factors involved in fibrosis and angiogenesis, promoting remodeling and cell growth.