This study was performed in accordance with the PHS Policy on Humane Care and Use of Laboratory Animals, the NIH Guide for the Care and Use of Laboratory Animals, and the Animal Welfare Act (7 U.S.C. et seq.); the animal use protocol was approved by the Institutional Animal Care and Use Committees (IACUC) of University of Wisconsin and William S. Middleton VA Hospital.
Article first published online: 2 JAN 2009
Copyright © 2008 The Triological Society
Volume 118, Issue 3, pages 559–563, March 2008
How to Cite
Russell, J. A., Nagai, H. and Connor, N. P. (2008), Effect of Aging on Blood Flow in Rat Larynx. The Laryngoscope, 118: 559–563. doi: 10.1097/MLG.0b013e31815bac17
This work was supported by research grants from the National Institute of Deafness and Other Communication Disorders (R01DC005935; R01DC008149).
- Issue published online: 2 JAN 2009
- Article first published online: 2 JAN 2009
- Manuscript Accepted: 19 SEP 2007
- blood flow;
- in vivo model
Objective/Hypothesis: Age-associated muscular changes and fatigue have been shown to affect phonatory function. Reductions in blood flow with aging could translate to reductions in oxidative capacity within laryngeal muscles and increased fatigability. We tested the hypothesis that there would be increased capillary red blood cell (RBC) velocity and a reduction of capillary density in the thyroarytenoid (TA) muscle of senescent rats.
Study Design/Methods: Ten male Fisher 344/Brown Norway rats in two age groups were used: young adult (9 mo) and old (28–30 mo). Sixteen additional young and old rats were used in a fluorescent microsphere experiment that examined blood flow rates before and after a surgical manipulation. With use of a specially equipped intravital microscope, in vivo measurements of capillary geometry and flow were obtained, including RBC velocity, capillary density, tortuosity, and number of branch points.
Results: There was an age-related reduction in capillary surface area as evidenced by reduced lineal density of capillaries. In addition, reduced RBC transit time was suggested by the reduction in branch points found with age. There was no change in RBC velocity with aging. The surgical method used to expose the TA muscle for blood flow recordings did not significantly affect resultant blood flow measurements.
Conclusions: We developed a method to evaluate in vivo laryngeal microvasculature. We found age-related changes in microvascular geometry within the TA muscle of the rat that could affect blood flow to this critical muscle of phonation and airway protection. These microvascular changes could contribute to age-related laryngeal dysfunction.