Editor's Note: This Manuscript was accepted for publication April 15, 2008. This work was supported by grants from Hungarian Scientific Research Fund (OTKA K63743).
Disease-Associated Novel CD46 Splicing Variants and Pathologic Bone Remodeling in Otosclerosis†
Article first published online: 2 JAN 2009
Copyright © 2008 The Triological Society
Volume 118, Issue 9, pages 1669–1676, September 2008
How to Cite
Karosi, T., Szalmás, A., Csomor, P., Kónya, J., Petkó, M. and Sziklai, I. (2008), Disease-Associated Novel CD46 Splicing Variants and Pathologic Bone Remodeling in Otosclerosis. The Laryngoscope, 118: 1669–1676. doi: 10.1097/MLG.0b013e31817c133d
- Issue published online: 2 JAN 2009
- Article first published online: 2 JAN 2009
- measles virus;
- bone remodeling;
- alternative splicing
Objective/Hypothesis: Otosclerotic bone is supposed to show unique CD46 expression pattern because otosclerosis is an organ-specific disease with viral etiology.
Study Design: Otosclerosis is a complex bone remodeling disorder of the human otic capsule, which is associated with persisting measles virus infection. The general cellular receptor of measles virus is the CD46, which has 14-known splicing isoforms.
Methods: Nucleic acid was extracted from ankylotic stapes footplates (N = 99) removed during stapedectomies. Consecutive histological, CD46 specific immunohistologic analysis, and multiple polymerase chain reaction (PCR) amplifications were performed. Measles virus was detected by seminested reverse transcriptase-PCR. Splicing variants of CD46 were identified by nested reverse transcriptase-PCR and finally determined by mass sequencing of complementary DNA.
Results: Measles virus RNA was detectable only in histologically otosclerotic stapes footplates. Virus negative-fixed stapes represent degenerative disorders of variable histopathology. Otosclerosis is featured by an increased number of osteoclasts showing strong CD46 immunoreaction in contrast to nonotosclerotic stapes fixations. Normal and nonotosclerotic stapes footplates show consistent expression of “c,” “d,” “e,” “f,” and “l” CD46 splicing isoforms. In contrast, four novel CD46 splicing variants were additionally detected in otosclerosis: os1, os2, os3, and os4.
Conclusions: Newly described CD46 isoforms have shorter or missing transmembrane domain and a rare cytoplasmic tail with pathological or uncommon signal transduction; however, virus binding ability remains equal and invariable. These changes may be responsible for the smooth virus replication. A special expression pattern and altered functions of CD46 could explain the organ-specific and virus-associated pathogenesis of otosclerosis.