Chapter 10.1 Introduction to cryocrystallography

Crystallography of biological macromolecules

First Online Edition (2006)

Part 10. Cryocrystallography

  1. H. Hope

Published Online: 1 JAN 2006

DOI: 10.1107/97809553602060000672

International Tables for Crystallography

International Tables for Crystallography

How to Cite

Hope, H. 2006. Introduction to cryocrystallography. International Tables for Crystallography. F:10:10.1:197–201.

Author Information

  1. Department of Chemistry, University of California, Davis, One Shields Ave, Davis, CA 95616-5295, USA

Publication History

  1. Published Online: 1 JAN 2006


A general introduction to cryocrystallography is provided. The chapter covers crystal mounting, cooling of biocrystals, principles of cooling equipment and frost prevention. Operational considerations, such as dual-stream instruments, electrically heated nozzles, temperature calibration and transfer of crystals to difractometers, are also described.


  • cryocrystallography;
  • crystal mounting;
  • data collection;
  • frost prevention;
  • ice formation;
  • radiation damage