Chapter

Chapter 12.1 The preparation of heavy-atom derivatives of protein crystals for use in multiple isomorphous replacement and anomalous scattering

Crystallography of biological macromolecules

First Online Edition (2006)

Part 12. Isomorphous replacement

  1. D. Carvin1,
  2. S. A. Islam2,
  3. M. J. E. Sternberg2,
  4. T. L. Blundell3

Published Online: 1 JAN 2006

DOI: 10.1107/97809553602060000679

International Tables for Crystallography

International Tables for Crystallography

How to Cite

Carvin, D., Islam, S. A., Sternberg, M. J. E. and Blundell, T. L. 2006. The preparation of heavy-atom derivatives of protein crystals for use in multiple isomorphous replacement and anomalous scattering. International Tables for Crystallography. F:12:12.1:247–255.

Author Information

  1. 1

    Biomolecular Modelling Laboratory, Imperial Cancer Research Fund, 44 Lincoln’s Inn Field, London WC2A 3PX, England

  2. 2

    Institute of Cancer Research, 44 Lincoln’s Inn Fields, London WC2A 3PX, England

  3. 3

    Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge, CB2 1GA, England

Publication History

  1. Published Online: 1 JAN 2006

Abstract

In this chapter an introduction to the heavy-atom data bank is provided and strategies are reviewed that can be adopted in the preparation of heavy-atom derivatives of protein crystals for use in multiple isomorphous replacement and anomalous scattering. Topics covered include: heavy-atom derivatives and their complexes; amino acids as ligands; the protein chemistry of heavy-atom reagents; metal-ion replacement in metalloproteins; analogues of amino acids; and the use of the heavy-atom data bank to select derivatives.

Keywords:

  • amino-acid analogues as heavy-atom derivatives;
  • anomalous scattering;
  • heavy-atom data bank;
  • heavy-atom derivatives;
  • heavy-atom reagents;
  • isomorphous replacement;
  • multiple isomorphous replacement