Chapter 19.6 Electron cryomicroscopy

Crystallography of biological macromolecules

First Online Edition (2006)

Part 19. Other experimental techniques

  1. T. S. Baker1,
  2. R. Henderson2

Published Online: 1 JAN 2006

DOI: 10.1107/97809553602060000703

International Tables for Crystallography

International Tables for Crystallography

How to Cite

Baker, T. S. and Henderson, R. 2006. Electron cryomicroscopy. International Tables for Crystallography. F:19:19.6:451–463.

Author Information

  1. 1

    Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907-1392, USA

  2. 2

    Medical Research Council, Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, England

Publication History

  1. Published Online: 1 JAN 2006


This chapter gives a complete but concise introduction to electron cryomicroscopy. The theoretical and practical background is discussed together with a review of applications to the three-dimensional structural analysis of biological macromolecules or macromolecular assemblies. It includes basic descriptions of how to analyse the structures of molecules arranged in the form of two-dimensional crystals, helical arrays or as single particles with or without symmetry. The chapter concludes by anticipating trends towards increased automation, development of better electronic cameras and increasing use of electron tomography for analysis of cell structure.


  • cryo EM;
  • electron cryomicroscopy;
  • electron microscopy;
  • electron scattering;
  • helical particles;
  • icosahedral particles;
  • image processing in cryo EM;
  • phasing;
  • radiation damage;
  • three-dimensional image reconstruction;
  • two-dimensional crystals