Chapter

Chapter 19.4 Small-angle neutron scattering

Crystallography of biological macromolecules

Second Online Edition (2012)

Part 19. Other experimental techniques

  1. D. M. Engelman1,
  2. P. B. Moore2

Published Online: 14 APR 2012

DOI: 10.1107/97809553602060000870

International Tables for Crystallography

International Tables for Crystallography

How to Cite

Engelman, D. M. and Moore, P. B. 2012. Small-angle neutron scattering. International Tables for Crystallography. F:19:19.4:575–582.

Author Information

  1. 1

    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520, USA

  2. 2

    Departments of Chemistry and Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520, USA

Publication History

  1. Published Online: 14 APR 2012

Abstract

The use of small-angle neutron scattering (SANS) for the study of biological macromolecules is described. Topics covered in this chapter include: the theoretical basis of SANS; contrast variation; and the measurement of distances between chemically specified points in a macromolecule or macromolecular complex. Practical considerations are addressed and a number of examples are provided.

Keywords:

  • contrast matching;
  • contrast variation;
  • distance measurements;
  • internal contrast variation;
  • isotopic substitution;
  • molecular weight;
  • small-angle neutron scattering;
  • spin contrast variation;
  • triple isotopic substitution