Chapter

Chapter 19.8 Use of SPIDER and SPIRE in image reconstruction

Crystallography of biological macromolecules

Second Online Edition (2012)

Part 19. Other experimental techniques

  1. A. Leith1,
  2. W. Baxter1,
  3. J. Frank2,3

Published Online: 14 APR 2012

DOI: 10.1107/97809553602060000874

International Tables for Crystallography

International Tables for Crystallography

How to Cite

Leith, A., Baxter, W. and Frank, J. 2012. Use of SPIDER and SPIRE in image reconstruction. International Tables for Crystallography. F:19:19.8:620–623.

Author Information

  1. 1

    Resource for Visualization of Biological Complexity, Wadsworth Center, Empire State Plaza, Albany, NY 12201–0509, USA

  2. 2

    Department of Biochemistry and Molecular Biophysics, Columbia University, 650 West 168th Street, NY 10032, USA

  3. 3

    Department of Biological Sciences, Columbia University, 650 West 168th Street, NY 10032, USA

Publication History

  1. Published Online: 14 APR 2012

Abstract

SPIDER is a comprehensive command-operated suite of programs for image processing in electron microscopy, with special emphasis on single-particle reconstruction. This chapter provides an outline of the way SPIDER and SPIRE, its interactive graphical user interface, may be used to obtain a three-dimensional reconstruction from projections of many randomly oriented realizations of a biological molecule.

Keywords:

  • single-particle reconstruction;
  • SPIDER;
  • SPIRE;
  • image processing;
  • electron microscopy