Chapter 19.8 Use of SPIDER and SPIRE in image reconstruction
Crystallography of biological macromolecules
Second Online Edition (2012)
Part 19. Other experimental techniques
Published Online: 14 APR 2012
© International Union of Crystallography 2006
International Tables for Crystallography
How to Cite
Leith, A., Baxter, W. and Frank, J. 2012. Use of SPIDER and SPIRE in image reconstruction. International Tables for Crystallography. F:19:19.8:620–623.
- Published Online: 14 APR 2012
SPIDER is a comprehensive command-operated suite of programs for image processing in electron microscopy, with special emphasis on single-particle reconstruction. This chapter provides an outline of the way SPIDER and SPIRE, its interactive graphical user interface, may be used to obtain a three-dimensional reconstruction from projections of many randomly oriented realizations of a biological molecule.
- single-particle reconstruction;
- image processing;
- electron microscopy