Chapter 19.10 Single-particle reconstruction with EMAN
Crystallography of biological macromolecules
Second Online Edition (2012)
Part 19. Other experimental techniques
Published Online: 14 APR 2012
© International Union of Crystallography 2006
International Tables for Crystallography
How to Cite
Ludtke, S. 2012. Single-particle reconstruction with EMAN. International Tables for Crystallography. F:19:19.10:629–632.
- Published Online: 14 APR 2012
Single-particle reconstruction is a technique for determining the three-dimensional structure of large sets of identical nanoscale objects, typically proteins or macromolecular assemblies, using electron cryomicroscopy (cryo-EM). This technique has now demonstrated resolutions of 3–5 Å for a number of targets and can routinely achieve subnanometre resolution. EMAN is a scientific image-processing software suite first developed to make single-particle reconstructions more reproducible and less labour-intensive. While it has since expanded to include many algorithms for other types of image processing, providing robust single-particle reconstructions with minimal effort remains one of its primary focuses. It includes a complete workflow infrastructure for this and other tasks in cryo-EM image analysis and structural biology.
- single-particle reconstruction;
- electron cryomicroscopy