Chapter

Chapter 19.10 Single-particle reconstruction with EMAN

Crystallography of biological macromolecules

Second Online Edition (2012)

Part 19. Other experimental techniques

  1. S. Ludtke

Published Online: 14 APR 2012

DOI: 10.1107/97809553602060000876

International Tables for Crystallography

International Tables for Crystallography

How to Cite

Ludtke, S. 2012. Single-particle reconstruction with EMAN. International Tables for Crystallography. F:19:19.10:629–632.

Author Information

  1. National Center for Macromolecular Imaging, Department of Biochemistry and Molecular Biology, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA

Publication History

  1. Published Online: 14 APR 2012

Abstract

Single-particle reconstruction is a technique for determining the three-dimensional structure of large sets of identical nanoscale objects, typically proteins or macromolecular assemblies, using electron cryomicroscopy (cryo-EM). This technique has now demonstrated resolutions of 3–5 Å for a number of targets and can routinely achieve subnanometre resolution. EMAN is a scientific image-processing software suite first developed to make single-particle reconstructions more reproducible and less labour-intensive. While it has since expanded to include many algorithms for other types of image processing, providing robust single-particle reconstructions with minimal effort remains one of its primary focuses. It includes a complete workflow infrastructure for this and other tasks in cryo-EM image analysis and structural biology.

Keywords:

  • EMAN;
  • single-particle reconstruction;
  • electron cryomicroscopy