Crystallization and preliminary X-ray diffraction analysis of β-cinnamomin, an elicitin secreted by the phytopathogenic fungus Phytophthora cinnamomin
Acta Crystallographica Section D
Volume 56, Issue 3, pages 363–365, March 2000
How to Cite
Archer, M., Rodrigues, M. L., Aurélio, M., Biemans, R., Cravador, A. and Carrondo, M. A. (2000), Crystallization and preliminary X-ray diffraction analysis of β-cinnamomin, an elicitin secreted by the phytopathogenic fungus Phytophthora cinnamomin. Acta Crystallographica Section D, 56: 363–365. doi: 10.1107/S0907444900000366
- phytopathogenic fungi.
Cinnamomin (CIN) belongs to a family of 10 kDa proteins designated as elicitins. Some of these proteins induce a hypersensitive response in diverse plant species, leading to resistance against fungal and bacterial plant pathogens. CIN was crystallized by the vapour-diffusion method using either ammonium sulfate or polyethyleneglycol (PEG) as precipitants in solutions buffered at around pH 7. These crystals are isomorphous and belong to the triclinic space group, with unit-cell parameters a = 31.69, b = 36.99, c = 44.09 Å, α = 76.86, β = 84.41, γ = 80.26°. A frozen crystal diffracted X-rays beyond 1.45 Å resolution on a synchrotron-radiation source.