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Keywords:

  • invertases;
  • pectin methylesterase;
  • post-translational regulation;
  • plant enzymes;
  • carbohydrate metabolism;
  • Nicotiana tabacum

Acid invertases catalyzing the breakdown of sucrose are regulated at the post-translational level by extracellular inhibitory proteins of 16–­20 kDa molecular weight in a pH-dependent manner. Little is known about the characteristics of the underlying protein–protein interaction. Here, the expression, purification, characterization, crystallization and initial X-ray analysis of a biologically active invertase inhibitor Nt-CIF from tobacco is reported. Four crystal forms covering a wide pH range have been obtained and data sets at resolutions higher than 2.5 Å have been collected.