Structure of Escherichia coli ribonucleotide reductase R2 in space group P6122
Acta Crystallographica Section D
Volume 61, Issue 12, pages 1649–1654, December 2005
How to Cite
Sommerhalter, M., Saleh, L., Bollinger, J. M. and Rosenzweig, A. C. (2005), Structure of Escherichia coli ribonucleotide reductase R2 in space group P6122. Acta Crystallographica Section D, 61: 1649–1654. doi: 10.1107/S0907444905034062
- ribonucleotide reductase R2;
- diiron center;
- dimanganese center;
- crystal contacts.
A new crystal form of wild-type ribonucleotide reductase R2 from Escherichia coli was obtained. Crystals grow in space group P6122 with one R2 monomer in the asymmetric unit. A twofold crystallographic symmetry axis generates the physiological dimeric form of R2. Co-crystallization with CoCl2 or MnCl2 results in full occupancy of the dinuclear metal site. The structure of the MnII-loaded form was determined to 2.6 Å resolution by molecular replacement. The crystallization conditions, backbone conformation, crystal-packing interactions and metal centers are compared with those of previously determined crystal forms.