The Buccaneer software for automated model building. 1. Tracing protein chains
Acta Crystallographica Section D
Volume 62, Issue 9, pages 1002–1011, September 2006
How to Cite
Cowtan, K. (2006), The Buccaneer software for automated model building. 1. Tracing protein chains. Acta Crystallographica D, 62: 1002–1011. doi: 10.1107/S0907444906022116
- model building;
- tracing protein chains.
A new technique for the automated tracing of protein chains in experimental electron-density maps is described. The technique relies on the repeated application of an oriented electron-density likelihood target function to identify likely Cα positions. This function is applied both in the location of a few promising `seed' positions in the map and to grow those initial Cα positions into extended chain fragments. Techniques for assembling the chain fragments into an initial chain trace are discussed.