SEARCH

SEARCH BY CITATION

Keywords:

  • Buccaneer;
  • model building;
  • tracing protein chains.

A new technique for the automated tracing of protein chains in experimental electron-density maps is described. The technique relies on the repeated application of an oriented electron-density likelihood target function to identify likely Cα positions. This function is applied both in the location of a few promising `seed' positions in the map and to grow those initial Cα positions into extended chain fragments. Techniques for assembling the chain fragments into an initial chain trace are discussed.