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A binary complex of dihydropteridine reductase and NADH crystallizes in the space group C2, with a = 222.2, b = 46.5, c = 95.3 Å and β = 101.1°. There are two dimers in the asymmetric unit. The structure was solved by molecular-replacement techniques and refined with 2.6 Å data to a crystallographic R factor of 16.8%. Each dimer has twofold non-crystallographic symmetry and the four individual monomers in the asymmetric unit have the same overall molecular conformation.