Crystallization and preliminary X-ray studies of mouse tumor necrosis factor
Acta Crystallographica Section D
Volume 53, Issue 3, pages 329–330, May 1997
How to Cite
Baeyens, K. J., De Bondt, H. L., Raeymaekers, A., De Ranter, C. J. and Fiers, W. (1997), Crystallization and preliminary X-ray studies of mouse tumor necrosis factor. Acta Crystallographica D, 53: 329–330. doi: 10.1107/S090744499601534X
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Well diffracting crystals of recombinant mouse tumor necrosis factor (mTNF) have been obtained. The sitting-drop vapor-diffusion method was used to grow crystals suitable for X-ray studies from solutions containing methoxypolyethylene glycol 2000 and isopropanol as precipitants. The crystals belong to the space group P1 with unit-cell dimensions a = 49.40, b = 48.24, c = 51.13 Å, α = 115.06, β = 103.32, γ = 91.27° and one trimer in the asymmetric unit. Crystals are stable to X-rays and diffract beyond 2.0 Å. Cooling the crystal during data collection is necessary since crystals dissolve at room temperature.