Quaternary Structure of UEA-II, the Chitobiose Specific Lectin from Gorse
Acta Crystallographica Section D
Volume 54, Issue 5, pages 844–847, September 1998
How to Cite
Dao-Thi, M.-H., Rizkallah, P., Wyns, L., Poortmans, F. and Loris, R. (1998), Quaternary Structure of UEA-II, the Chitobiose Specific Lectin from Gorse. Acta Cryst. D, 54: 844–847. doi: 10.1107/S0907444998001218
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The chitobiose specific Ulex europaeus lectin II crystallizes in space group P3221 with unit-cell dimensions a = b = 105.54, c = 176.26 Å. The asymmetric unit contains a complete lectin tetramer. The crystals were shown to diffract to 4.5 Å on a rotating-anode source and to 2.7 Å at the Daresbury synchrotron source. Molecular replacement and subsequent rigid-body refinement using data to 4.5 Å yielded a solution corresponding to a tetramer very similar to that of phytohemagglutinin-L and soybean agglutinin. The monomers in the Ulex lectin tetramer are rotated ∼ 5° compared with the phytohemagglutinin-L and soybean agglutinin structures.