Experimental procedure for the characterization of radiation damage in macromolecular crystals
International Union of Crystallography, 2011
Journal of Synchrotron Radiation
Volume 18, Issue 3, pages 381–386, May 2011
How to Cite
Leal, R. M. F., Bourenkov, G. P., Svensson, O., Spruce, D., Guijarro, M. and Popov, A. N. (2011), Experimental procedure for the characterization of radiation damage in macromolecular crystals. Jnl of Synchrotron Radiation, 18: 381–386. doi: 10.1107/S0909049511002251
- radiation damage
A reliable and reproducible method to automatically characterize the radiation sensitivity of macromolecular crystals at the ESRF beamlines has been developed. This new approach uses the slope of the linear dependence of the overall isotropic B-factor with absorbed dose as the damage metric. The method has been implemented through an automated procedure using the EDNA on-line data analysis framework and the MxCuBE data collection control interface. The outcome of the procedure can be directly used to design an optimal data collection strategy. The results of tests carried out on a number of model and real-life crystal systems are presented.