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Keywords:

  • l-N-carbamoylases;
  • N-carbamoyl-l-amino-acid amidohydrolases;
  • hydantoinase process;
  • peptidase family

N-Carbamoyl-l-amino-acid amidohydrolases (l-N-carbamoylases; EC 3.5.1.87) hydrolyze the carbon–nitrogen bond of the ureido group in N-carbamoyl-l-α-amino acids. These enzymes are commonly used in the production of optically pure natural and non-natural l-amino acids using the `hydantoinase process'. Recombinant l-N-carbamoylase from Geobacillus stearothermophilus CECT43 has been expressed, purified and crystallized by hanging-drop vapour diffusion. X-ray data were collected to a resolution of 2.75 Å. The crystals belonged to space group P21212, with unit-cell parameters a = 103.2, b = 211.7, c = 43.1 Å and two subunits in the asymmetric unit.