Purification, crystallization and preliminary crystallographic analysis of the catalytic domain of the extracellular cellulase CBHI from Trichoderma harzianum
International Union of Crystallography, 2010
Acta Crystallographica Section F
Volume 66, Issue 9, pages 1041–1044, September 2010
How to Cite
Colussi, F., Textor, L. C., Serpa, V., Maeda, R. N., Pereira, N. and Polikarpov, I. (2010), Purification, crystallization and preliminary crystallographic analysis of the catalytic domain of the extracellular cellulase CBHI from Trichoderma harzianum. Acta Crystallographica Section F, 66: 1041–1044. doi: 10.1107/S1744309110026886
- Trichoderma harzianum;
The filamentous fungus Trichoderma harzianum has a considerable cellulolytic activity that is mediated by a complex of enzymes which are essential for the hydrolysis of microcrystalline cellulose. These enzymes were produced by the induction of T. harzianum with microcrystalline cellulose (Avicel) under submerged fermentation in a bioreactor. The catalytic core domain (CCD) of cellobiohydrolase I (CBHI) was purified from the extracellular extracts and submitted to robotic crystallization. Diffraction-quality CBHI CCD crystals were grown and an X-ray diffraction data set was collected under cryogenic conditions using a synchrotron-radiation source.