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Disulfide structure of alfimeprase: A recombinant analog of fibrolase

  1. Geoff Jones,
  2. Michael Ronk,
  3. Faith Mori,
  4. Zhongqi Zhang*

Article first published online: 31 DEC 2008

DOI: 10.1110/ps.110101

Issue

Protein Science

Protein Science

Volume 10, Issue 6, pages 1264–1267, June 2001

Additional Information

How to Cite

Jones, G., Ronk, M., Mori, F. and Zhang, Z. (2001), Disulfide structure of alfimeprase: A recombinant analog of fibrolase. Protein Science, 10: 1264–1267. doi: 10.1110/ps.110101

Author Information

  1. Analytical Research & Development Department, Amgen Inc., Thousand Oaks, California 91320, USA

*Dr. Zhongqi Zhang, Amgen Inc., M/S 25–2-A, One Amgen Center Drive, Thousand Oaks, CA 91320, USA; fax: 805-447-8690.

Publication History

  1. Issue published online: 31 DEC 2008
  2. Article first published online: 31 DEC 2008
  3. Manuscript Revised: 16 MAR 2001
  4. Manuscript Accepted: 16 MAR 2001
  5. Manuscript Received: 2 JAN 2001

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Keywords:

  • Fibrolase;
  • alfimeprase;
  • disulfide bond;
  • Edman degradation;
  • mass spectrometry

Abstract

The disulfide structure of alfimeprase, a recombinant analog of fibrolase, was experimentally determined by a combination of peptide mapping, Edman degradation, and mass spectrometry. The three disulfide bonds were determined to be Cys-116/196, Cys-156 /180, and Cys-158/163 with the residue number system of alfimeprase.

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