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Evaluation of the energetic contribution of an ionic network to beta-sheet stability

  1. Kirsten S. Lassila1,
  2. Deepshikha Datta1,
  3. Stephen L. Mayo2,*

Article first published online: 13 APR 2009

DOI: 10.1110/ps.23502

Issue

Protein Science

Protein Science

Volume 11, Issue 3, pages 688–690, March 2002

Additional Information

How to Cite

Lassila, K. S., Datta, D. and Mayo, S. L. (2002), Evaluation of the energetic contribution of an ionic network to beta-sheet stability. Protein Science, 11: 688–690. doi: 10.1110/ps.23502

Author Information

  1. 1

    Biochemistry Option, California Institute of Technology, MC 147–75, Pasadena, California 91125, USA

  2. 2

    Howard Hughes Medical Institute and Divisions of Biology and Chemistry, California Institute of Technology, MC 147–75, Pasadena, California 91125, USA

*Howard Hughes Medical Institute, Divisions of Biology and Chemistry, California Institute of Technology, MC 147-75, Pasadena, CA 91125, USA; fax: (626) 568-0934.

Publication History

  1. Issue published online: 13 APR 2009
  2. Article first published online: 13 APR 2009
  3. Manuscript Accepted: 30 NOV 2001
  4. Manuscript Received: 19 JUN 2001

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Keywords:

  • β-Sheet;
  • protein stability;
  • protein design;
  • electrostatics;
  • side chain interactions

Abstract

We have evaluated the interaction energy of a three-residue ionic network constructed on the β-sheet surface of protein G using double mutant cycles. Although the two individual ion pairs were each stabilizing by ∼0.6 kcal/mol, the excess gain in stability for the triad was small (0.06 kcal/mol).

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