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jde12076-sup-0001-FigS1.tifimage/tif1147KFigure S1. Double immunostaining with anti-epiplakin (EPPK) antibodies (green) and anti-keratin 8/18 (a, red) or anti-vimentin (b, red) antibodies.(a; stained also with 4′,6′-diamidino-2-phenylindole dihydrochloride) Endogeneous EPPK co-localized with Intermediate Filaments network and it co-localized more closely with the keratin network than with vimentin in HeLa cells. Bar, 10 μm.
jde12076-sup-0002-FigS2.tifimage/tif993KFigure S2. We stained HeLa cell clusters in 3-D culture in Matrigel using anti-BP230 antibodies (sc13777). One of the hemidesmosomal proteins, BP230, not only co-localized with epiplakin (EPPK) partly on the outermost surface of the cell clusters, but also located inside cell clusters.

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