In vitro responses of neurofibroma fibroblasts, mast cells and Schwann cells obtained from patients with neurofibromatosis 1 to 308-nm excimer light and/or vitamin D3

Authors


Correspondence: Juichiro Nakayama, M.D., Ph.D., Department of Dermatology, Fukuoka University Faculty of Medicine, 7-45-1 Nanakuma, Jonan-ku, Fukuoka 814-0180, Japan. Email: dermatol@fukuoka-u.ac.jp

Abstract

Fibroblasts, mast cells and Schwann cells were isolated from neurofibromas of patients with neurofibromatosis 1, and their responses to 308-nm excimer light irradiation and/or vitamin D3 or an analog (tacalcitol; 1,24-dihydroxyvitamin D3) were examined in vitro. Excimer light irradiation (300 mJ/cm2) suppressed the growth of all three cell types. Exposure to 107 mol/L of 1α,25(OH)2D3 (VD3) or tacalcitol suppressed the growth of fibroblasts and mast cells, but not Schwann cells. These results suggest that the different neurofibroma cell types show different responses to VD3. A combination of excimer light irradiation and VD3 is necessary to suppress the growth of neurofibroma cells in vivo.

Ancillary