Rapid detection of human cytomegalovirus UL97 and UL54 mutations for antiviral resistance in clinical specimens



Kimiyasu Shiraki, Department of Virology, University of Toyama, 2630 Sugitani, Toyama 930-0194, Japan.

Tel: +81 76 434 7255; fax: +81 76 434 5020; email: kshiraki@med.u-toyama.ac.jp


Drug-resistant cytomegalovirus appears during prolonged anti-cytomegalovirus therapy. Assays for human cytomegalovirus viral protein kinase (UL97) and viral DNA polymerase (UL54) gene mutations conferring drug resistance have been used rather than susceptibility assays to assess clinical specimens. In this study a sensitive system for genotype assay of UL97 and UL54 in clinical specimens with as few as six copies/µg of DNA was developed.