Anthocyanins from black soybean inhibit Helicobacter pylori-induced inflammation in human gastric epithelial AGS cells

Authors

  • Jung-Min Kim,

    1. Department of Microbiology, Gyeongsang National University School of Medicine, Gyeongsang Institute of Health Science, Jinju, South Korea
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  • Kyung-Mi Kim,

    1. Department of Microbiology, Gyeongsang National University School of Medicine, Gyeongsang Institute of Health Science, Jinju, South Korea
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  • En-Hee Park,

    1. Department of Microbiology, Gyeongsang National University School of Medicine, Gyeongsang Institute of Health Science, Jinju, South Korea
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  • Ji-Hyun Seo,

    1. Department of Pediatrics, Gyeongsang National University School of Medicine, Gyeongsang Institute of Health Science, Jinju, South Korea
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  • Jae-Young Song,

    1. Department of Microbiology, Gyeongsang National University School of Medicine, Gyeongsang Institute of Health Science, Jinju, South Korea
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  • Sung-Chul Shin,

    1. Department of Chemistry, Gyeongsang National University, Jinju, South Korea
    2. Research Institute of Life Science, Gyeongsang National University, Jinju, South Korea
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  • Hyung-Lyun Kang,

    1. Department of Microbiology, Gyeongsang National University School of Medicine, Gyeongsang Institute of Health Science, Jinju, South Korea
    2. Research Institute of Life Science, Gyeongsang National University, Jinju, South Korea
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  • Woo-Kon Lee,

    1. Department of Microbiology, Gyeongsang National University School of Medicine, Gyeongsang Institute of Health Science, Jinju, South Korea
    2. Research Institute of Life Science, Gyeongsang National University, Jinju, South Korea
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  • Myung-Je Cho,

    1. Department of Microbiology, Gyeongsang National University School of Medicine, Gyeongsang Institute of Health Science, Jinju, South Korea
    2. Research Institute of Life Science, Gyeongsang National University, Jinju, South Korea
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  • Kwang-Ho Rhee,

    1. Department of Microbiology, Gyeongsang National University School of Medicine, Gyeongsang Institute of Health Science, Jinju, South Korea
    2. Research Institute of Life Science, Gyeongsang National University, Jinju, South Korea
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  • Hee-Shang Youn,

    1. Department of Pediatrics, Gyeongsang National University School of Medicine, Gyeongsang Institute of Health Science, Jinju, South Korea
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  • Seung-Chul Baik

    Corresponding author
    1. Research Institute of Life Science, Gyeongsang National University, Jinju, South Korea
    • Department of Microbiology, Gyeongsang National University School of Medicine, Gyeongsang Institute of Health Science, Jinju, South Korea
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Correspondence

Seung-Chul Baik, Department of Microbiology, Gyeongsang National University School of Medicine, 92 Chilam-Dong, Jinju 660-751, Korea. Tel: +82 55 772-8084; fax: +82 55 772 8089; e-mail: scbaik@gnu.ac.kr

ABSTRACT

Infection with Helicobacter pylori leads to gastritis, peptic ulcers and gastric cancer. Moreover, when the gastric mucosa is exposed to H. pylori, gastric mucosal inflammatory cytokine interleukin-8 (Il-8) and reactive oxygen species increase. Anthocyanins have anti-oxidative, antibacterial and anti-inflammatory properties. However, the effect of anthocyanins in H. pylori-infected cells is not yet clear. In this study, therefore, the effect of anthocyanins on H. pylori-infected human gastric epithelial cells was examined. AGS cells were pretreated with anthocyanins for 24 hrs followed by H. pylori 26695 infection for up to 24 hrs. Cell viability and ROS production were examined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and 2′,7′–dichlorofluorescein diacetate assay, respectively. Western blot analyses and RT-PCR were performed to assess gene and protein expression, respectively. IL-8 secretion in AGS cells was measured by ELISA. It was found that anthocyanins decrease H. pylori-induced ROS enhancement. Anthocyanins also inhibited phosphorylation of mitogen-activated protein kinases, translocation of nuclear factor-kappa B and Iκβα degradation. Furthermore anthocyanins inhibited H. pylori-induced inducible nitric oxide synthases and cyclooxygenase-2 mRNA expression and inhibited IL-8 production by 45.8%. Based on the above findings, anthocyanins might have an anti-inflammatory effect in H. pylori-infected gastric epithelial cells.

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