A bio-nanocapsule containing envelope protein domain III of Japanese encephalitis virus protects mice against lethal Japanese encephalitis virus infection

Authors

  • Takeshi Miyata,

    1. Molecular Microbiology Group, Department of Tropical Infectious Diseases, COMB, Tropical Biosphere Research Center, Japan
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    • Present address: Division of Molecular Functions of Food, Department of Biochemistry and Biotechnology, Kagoshima University, 1-21-24, Korimoto, Kagoshima, 890-0065, Japan.
  • Senji Tafuku,

    1. Molecular Microbiology Group, Department of Tropical Infectious Diseases, COMB, Tropical Biosphere Research Center, Japan
    2. Jectas Innovators Co. Ltd., 1 Senbaru, Nishihara, Okinawa, Japan
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  • Tetsuya Harakuni,

    1. Molecular Microbiology Group, Department of Tropical Infectious Diseases, COMB, Tropical Biosphere Research Center, Japan
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  • Masayuki Tadano,

    1. Department of Molecular Virology, Graduate School of Medicine, University of the Ryukyus, 207 Uehara, Nishihara, Okinawa, Japan
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  • Nobuo Yoshimoto,

    1. Graduate School of Bioagricultural Sciences, Nagoya University, Furo-Cho, Chikusa-Ku, Nagoya, Japan
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  • Masumi Iijima,

    1. Graduate School of Bioagricultural Sciences, Nagoya University, Furo-Cho, Chikusa-Ku, Nagoya, Japan
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  • Hidenori Matsuo,

    1. Graduate School of Bioagricultural Sciences, Nagoya University, Furo-Cho, Chikusa-Ku, Nagoya, Japan
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  • Goro Matsuzaki,

    1. Molecular Microbiology Group, Department of Tropical Infectious Diseases, COMB, Tropical Biosphere Research Center, Japan
    2. Division of Host Defense and Vaccinology, Department of Microbiology, Graduate School of Medicine, University of the Ryukyus, 1 Senbaru, Nishihara, Okinawa, Japan
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  • Shun'ichi Kuroda,

    1. Graduate School of Bioagricultural Sciences, Nagoya University, Furo-Cho, Chikusa-Ku, Nagoya, Japan
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  • Takeshi Arakawa

    Corresponding author
    1. Division of Host Defense and Vaccinology, Department of Microbiology, Graduate School of Medicine, University of the Ryukyus, 1 Senbaru, Nishihara, Okinawa, Japan
    • Molecular Microbiology Group, Department of Tropical Infectious Diseases, COMB, Tropical Biosphere Research Center, Japan
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Correspondence

Takeshi Arakawa, Division of Host Defense and Vaccinology, Department of Microbiology, Graduate School of Medicine, University of the Ryukyus, 1 Senbaru, Nishihara, Okinawa 903-0213, Japan.

Tel/fax: + 81 98 895 8974; email: tarakawa@comb.u-ryukyu.ac.jp

ABSTRACT

An engineered bio-nanocapsule (BNC) comprising modified hepatitis B surface antigen L protein was used as a physical scaffold for envelope protein domain III (D3) of Japanese encephalitis virus (JEV). At the N terminus, the BNC contained a two-tandem repeat of the Z domain (ZZ) derived from Staphylococcus aureus protein A (ZZ-BNC). The Lys-rich ZZ moiety exposed on the surface of ZZ-BNC was used for chemical conjugation with the JEV D3 antigen, which had been expressed and purified from Escherichia coli. Immunization of mice with D3 loaded on the surface of ZZ-BNC (ZZ-BNC:D3) augmented serum IgG response against JEV and increased protection against lethal JEV infection. The present study suggests that innocuous recombinant antigens, when loaded on the surface of ZZ-BNC, can be transformed to immunogenic antigens.

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