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mim12061-sm-0001-SupFig-S1.tif4407KFig. S1. Degradation of amino acids by N2 gas plasma. Twenty µL aliquots of 1 mg/mL solutions of various amino acids (alanine, aspartic acid, asparagine, histidine, tryptophan and tyrosine) were treated with N2 gas plasma at 1.5 kpps for 0, 5, 15 and 30 mins. An L-amino acid quantification kit (BioVision) was used. The quantity of intact amino acids was estimated by comparison of absorbance at 570 nm. For all amino acids, the absorbance value at 570 nm decreased after N2 gas plasma treatment. These observations suggest that N2 gas plasma treatment changes amino acids.
mim12061-sm-0002-SupFig-S2.tif3999KFig. S2. Coomassie brilliant blue (CBB) staining of N2 gas plasma treated BSA. BSA (100 µg) dried onto cover slips was subjected to N2 gas plasma treatment (1.5 kpps; 0, 7.5, 15 and 30 mins). The protein samples were analyzed by SDS––PAGE and transblotted onto a PVDF membrane, which was then stained with CBB. The intensity of the band corresponding to BSA (arrow, 66 kDa) decreased after N2 gas plasma treatment. This finding suggests degradation of BSA by N2 gas plasma treatment.

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