Identification of cellular factors required for the budding of koala retrovirus

Authors

  • Sayumi Shimode,

    1. Laboratory of Signal Transduction, Department of Cell Biology, Institute for Virus Research, Kyoto University, 53 Shogoin-Kawaharacho, Sakyo-ku, Kyoto, Japan
    Search for more papers by this author
    • These authors contributed equally to this study.
  • Rie Nakaoka,

    1. Department of Veterinary Pathology, School of Veterinary Medicine, Rakuno Gakuen University, 582 Bunkyodai-Midorimachi, Ebetsu, Hokkaido, Japan
    Search for more papers by this author
    • These authors contributed equally to this study.
  • Shigeki Hoshino,

    1. Laboratory of Signal Transduction, Department of Cell Biology, Institute for Virus Research, Kyoto University, 53 Shogoin-Kawaharacho, Sakyo-ku, Kyoto, Japan
    Search for more papers by this author
  • Masumi Abe,

    1. Fifth Biology Section for Microbiology, First Department of Forensic Science, National Research Institute of Police Science, 6-3-1 Kashiwanoha, Kashiwa, Chiba, Japan
    Search for more papers by this author
  • Hiroko Shogen,

    1. Laboratory of Signal Transduction, Department of Cell Biology, Institute for Virus Research, Kyoto University, 53 Shogoin-Kawaharacho, Sakyo-ku, Kyoto, Japan
    Search for more papers by this author
  • Jiro Yasuda,

    1. Fifth Biology Section for Microbiology, First Department of Forensic Science, National Research Institute of Police Science, 6-3-1 Kashiwanoha, Kashiwa, Chiba, Japan
    2. Department of Emerging Infectious Disease, Institute of Tropical Medicine, Nagasaki University, Nagasaki, Japan
    Search for more papers by this author
  • Takayuki Miyazawa

    Corresponding author
    • Laboratory of Signal Transduction, Department of Cell Biology, Institute for Virus Research, Kyoto University, 53 Shogoin-Kawaharacho, Sakyo-ku, Kyoto, Japan
    Search for more papers by this author

Correspondence

Takayuki Miyazawa, Laboratory of Signal Transduction, Department of Cell Biology, Institute for Virus Research, Kyoto University, 53 Shogoin-Kawaharacho, Sakyo-ku, Kyoto 606-8507, Japan.

Tel/fax: +81 75 751 4814; email: takavet@gmail.com

ABSTRACT

Koala retrovirus (KoRV) is a unique gammaretrovirus that is currently endogenizing into its host and considered to be associated with leukemia, lymphoma and immunosuppression in koalas (Phascolactos cinereus). In this study, it was demonstrated that WWP2 or WWP2-like E3 ubiquitin ligases possessing the WW domain closely related to WWP2 and Vps4A/B are involved in KoRV budding. These data suggest that KoRV Gag recruits the cellular endosomal sorting complex required for transport machinery through interaction of the PPPY L-domain with the WW domain(s) of WWP2 and that progeny virions are released from cells by utilizing the multivesicular body sorting pathway.

Ancillary