Myricetin suppresses lipoteichoic acid-induced interleukin-1β and cyclooxygenase-2 expression in human gingival fibroblasts

Authors

  • Gloria Gutiérrez-Venegas,

    Corresponding author
    1. Faculty of Dentistry, Division of Graduate Studies and Research, Laboratory of Biochemistry, National Autonomous University of Mexico, Federal District, Mexico D. F., Mexico
    • Correspondence

      Gloria Gutiérrez-Venegas, Faculty of Dentistry, Division of Graduate Studies and Research, Laboratory of Biochemistry, National Autonomous University of Mexico, Distrito Federal, Mexico. Tel/fax: +52 56225554; email: gloria@fo.odonto.unam.mx

    Search for more papers by this author
  • Oscar Alonso Luna,

    1. Faculty of Dentistry, Division of Graduate Studies and Research, Laboratory of Biochemistry, National Autonomous University of Mexico, Federal District, Mexico D. F., Mexico
    Search for more papers by this author
  • Jairo Agustín Ventura-Arroyo,

    1. Faculty of Dentistry, Division of Graduate Studies and Research, Laboratory of Biochemistry, National Autonomous University of Mexico, Federal District, Mexico D. F., Mexico
    Search for more papers by this author
  • Cristina Hernández-Bermúdez

    1. Faculty of Dentistry, Division of Graduate Studies and Research, Laboratory of Biochemistry, National Autonomous University of Mexico, Federal District, Mexico D. F., Mexico
    Search for more papers by this author

ABSTRACT

Periodontitis is an inflammatory disease affecting the connective tissue and supporting bone surrounding the teeth. In periodontitis, human gingival fibroblasts (HGFs) synthesize IL-1β, causing a progressive inflammatory response. Flavones demonstrate a variety of biological activity: among others, they possess anti-inflammatory properties. Myricetin is a flavone with a strong anti-inflammatory activity. The objective of this study was to evaluate the effect of the flavonoid myricetin on HGFs under inflammatory conditions induced by lipoteichoic acid (LTA). the effect of myricetin on HGFs was assessed by measuring cell viability, signaling pathways and IL-1β expression and synthesis. It was found that, over time, myricetin did not affect cell viability. However, it inhibited activation of p38 and extracellular-signal-regulated kinase-1/2 in LTA-treated HGFs and also blocked IκB degradation and cyclooxygenase-2 and prostaglandin E2 synthesis and expression. These findings suggest that myricetin has therapeutic effects in the form of controlling LTA-induced inflammatory responses.

Ancillary