Both these authors contributed equally to this study.
Improved protective efficacy of a chimeric Staphylococcus aureus vaccine candidate iron-regulated surface determinant B (N126–P361)-target of RNAIII activating protein in mice
Article first published online: 12 DEC 2013
© 2013 The Societies and Wiley Publishing Asia Pty Ltd
Microbiology and Immunology
Volume 57, Issue 12, pages 857–864, December 2013
How to Cite
Yu, L., Wang, N., Ma, J., Tong, C., Song, B., Chi, J., Ma, G., Zhu, Z. and Cui, Y. (2013), Improved protective efficacy of a chimeric Staphylococcus aureus vaccine candidate iron-regulated surface determinant B (N126–P361)-target of RNAIII activating protein in mice. Microbiology and Immunology, 57: 857–864. doi: 10.1111/1348-0421.12106
Present address: Ning Wang: Department of Medicine, Yaan Vocational College, Yaan 625000, China.
- Issue published online: 12 DEC 2013
- Article first published online: 12 DEC 2013
- Accepted manuscript online: 5 OCT 2013 04:21AM EST
- Manuscript Accepted: 26 SEP 2013
- Manuscript Revised: 2 SEP 2013
- Manuscript Received: 11 JUN 2013
- National Natural Science Foundation of China. Grant Number: 31072120
- Science and Technology Project for Heilongjiang Agricultural Reclamation Administration. Grant Number: HNK11A-08-01-04
- Chimeric vaccine;
- Staphylococcus aureus;
- target of RNAIII activating protein;
- truncated immunodominant antigen iron-regulated surface determinant B
The pathogen Staphylococcus aureus causes a wide range of serious infections, necessitating urgent development of a vaccine against this organism. However, currently developed vaccines are relatively ineffective because of the limited antigenic component that is contained in the vaccine formulations. To develop an effective S. aureus candidate vaccine, overlapping PCR was used to add the truncated immunodominant antigen iron-regulated surface determinant B (IsdB)(N126–P361) (tIsdB) to the N-terminal of intact antigen target of RNAIII activating protein (TRAP) and thus construct a tIsdB-TRAP chimera. The humoral and cellular immune responses against tIsdB-TRAP were compared with those against single or combined formulations. tIsdB-TRAP elicited significantly stronger humoral responses in mice (P < 0.05). As to cellular immune responses in mice, the tIsdB-TRAP group resulted in a greater IL-4 response than did other groups (P < 0.05). Greater amounts of IL-2 and IFN-γ were found in the tIsdB-TRAP group. Mouse challenge also showed that tIsdB-TRAP provided better protection against S. aureus than did the control groups. These results suggest that this chimeric protein may be a promising pathogen target for further vaccine development.