Activation of Toll-like receptor 9 inhibits lipopolysaccharide-induced receptor activator of nuclear factor kappa- B ligand expression in rat B lymphocytes

Authors

  • Xiaoqian Yu,

    1. Department of Immunology and Infectious Diseases, The Forsyth Institute, Cambridge, Massachusetts, USA
    2. Department of Periodontology, Peking University School and Hospital of Stomatology, Beijing
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  • Jiang Lin,

    1. Department of Immunology and Infectious Diseases, The Forsyth Institute, Cambridge, Massachusetts, USA
    2. Department of Stomatology, Fourth Hospital of Harbin Medical University, Harbin, China
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  • Qing Yu,

    1. Department of Immunology and Infectious Diseases, The Forsyth Institute, Cambridge, Massachusetts, USA
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  • Toshihisa Kawai,

    1. Department of Immunology and Infectious Diseases, The Forsyth Institute, Cambridge, Massachusetts, USA
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  • Martin A. Taubman,

    1. Department of Immunology and Infectious Diseases, The Forsyth Institute, Cambridge, Massachusetts, USA
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  • Xiaozhe Han

    Corresponding author
    1. Department of Immunology and Infectious Diseases, The Forsyth Institute, Cambridge, Massachusetts, USA
    • Correspondence

      Xiaozhe Han, Department of Immunology and Infectious Diseases, The Forsyth Institute, 245 First Street, Cambridge, Massachusetts 02142, USA. Tel: +1 617 892 8447; fax: +1 617 892 8612; email: xhan@forsyth.org

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ABSTRACT

B lymphocytes express multiple TLRs that regulate their cytokine production. We investigated the effect of TLR4 and TLR9 activation on receptor activator of NF-κB ligand (RANKL) expression by rat spleen B cells. Splenocytes or purified spleen B cells from Rowett rats were cultured with TLR4 ligand Escherichia coli LPS and/or TLR9 ligand CpG-oligodeoxynucleotide (CpG-ODN) for 2 days. RANKL mRNA expression and the percentage of RANKL-positive B cells were increased in rat splenocytes challenged by E. coli LPS alone. The increases were less pronounced when cells were treated with both CpG-ODN and E. coli LPS. Microarray analysis showed that expressions of multiple cyclin-dependent kinase (CDK) pathway-related genes were up-regulated only in cells treated with both E. coli LPS and CpG-ODN. This study suggests that CpG-ODN inhibits LPS-induced RANKL expression in rat B cells via regulation of the CDK pathway.

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