Levamisole enhances immunity in ducklings vaccinated against Riemerella anatipestifer

Authors

  • Yuewei Zhang,

    1. Key Laboratory of Rapid Diagnostic Technology for Animal Disease, Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, China
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    • Yuewei Zhang and Huiling Chen contributed equally to this work.
  • Huiling Chen,

    1. Beijing General Station of Animal Husbandry and Veterinary Service, Beijing Municipal Bureau of Agriculture, Beijing, China
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    • Yuewei Zhang and Huiling Chen contributed equally to this work.
  • Xiangfan Zeng,

    1. Key Laboratory of Rapid Diagnostic Technology for Animal Disease, Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, China
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  • Peng Wang,

    Corresponding author
    1. Animal Health Inspection Institute, Beijing, China
    • Correspondence

      Wenxue Wu, Key Laboratory of Rapid Diagnostic Technology for Animal Disease, Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, China. Tel: +86-10-6273-3048; fax: +86-10-6273-3048; email: wuwenxue@cau.edu.cn

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  • Jinxiang Li,

    1. Chinese Academy of Agricultural Sciences, Beijing, China
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  • Wenxue Wu

    1. Key Laboratory of Rapid Diagnostic Technology for Animal Disease, Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, China
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Abstract

Oil-adjuvant-inactivated vaccine is one of the most cost-effective vaccines used to protect ducklings against RA infection; however, it does not provide complete protection in very young ducklings with immature immune systems. In the current study, LMS was used as an immunopotentiator to improve the immune system in ducklings. Serum immunoglobulin (Ig)G titers and the secretions of both Th1-type (IFN-γ and IL-2) and Th2-type (IL-4 and IL-10) cytokines were higher in ducklings that had been vaccinated with LMS. In addition, a significantly higher T-lymphocyte proliferation rate was obtained with the addition of LMS. Furthermore, all of the ducklings vaccinated with LMS were protected against RA on the 9th day post-vaccination, whereas only 69.2% of the ducklings were protected in the group that did not receive LMS. These results suggest that LMS might be a useful adjuvant to enhance the immune response of ducklings. The use of LMS may also alleviate local injection lesions, caused by the oil-emulsion vaccine, by reducing the dose of the vaccine.

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