Polyadenylation of Friend murine leukemia virus env-mRNA is affected by its splicing

Authors

  • Akihito Machinaga,

    1. Department of Bioinformatics, Faculty of Engineering, Soka University, Hachioji-shi, Tokyo, Japan
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  • Sayaka Takase-Yoden

    Corresponding author
    1. Department of Bioinformatics, Faculty of Engineering, Soka University, Hachioji-shi, Tokyo, Japan
    • Correspondence

      Sayaka Takase-Yoden, Department of Bioinformatics, Faculty of Engineering, Soka University, 1-236, Tangi-machi, Hachioji-shi, Tokyo 192-8577, Japan. Tel: +81-42-691-2375; fax: +81-42-691-2375; email: takase@soka.ac.jp

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Abstract

As splicing was previously found to be important for increasing Friend murine leukemia virus env-mRNA stability and translation, we investigated whether splicing of env-mRNA affected the poly(A) tail length using env expression vectors that yielded unspliced or spliced env-mRNA. Incomplete polyadenylation was detected in a fraction of the unspliced env-mRNA products in an env gene-dependent manner, showing that splicing of Friend murine leukemia virus plays an important role in the efficiency of complete polyadenylation of env-mRNA. These results suggested that the promotion of complete polyadenylation of env-mRNA by splicing might partially explain up-regulation of Env protein expression as a result of splicing.

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