Inhibition of pilocarpine-induced saliva secretion by adrenergic agonists in ICR mice
Article first published online: 26 NOV 2012
© 2012 The Authors Clinical and Experimental Pharmacology and Physiology © 2012 Wiley Publishing Asia Pty Ltd
Clinical and Experimental Pharmacology and Physiology
Volume 39, Issue 12, pages 1038–1043, December 2012
How to Cite
Imamura, T. K., Yoshino, Y., Yamachika, S., Ishii, H., Watanabe, N. Y., Inoue, H. and Nakagawa, Y. (2012), Inhibition of pilocarpine-induced saliva secretion by adrenergic agonists in ICR mice. Clinical and Experimental Pharmacology and Physiology, 39: 1038–1043. doi: 10.1111/1440-1681.12023
- Issue published online: 26 NOV 2012
- Article first published online: 26 NOV 2012
- Accepted manuscript online: 17 OCT 2012 11:16PM EST
- Manuscript Accepted: 15 OCT 2012
- Manuscript Revised: 11 OCT 2012
- Manuscript Received: 10 FEB 2012
- Scientific Research . Grant Number: 17592116
- aquaporin 5;
- autonomic nerve;
- The aim of the present study was to clarify the effects of the adrenoceptor agonist isoproterenol (IPR) on saliva secretion stimulated by the muscarinic receptor agonist pilocarpine (PILO) in mice.
- Mice were injected with either 0.5 mg/kg, i.p. PILO alone or simultaneously with 2 mg/kg, i.p., IPR to evaluate the inhibitory effects of adrenoceptor agonists on saliva secretion. The mechanisms underlying changes in saliva flow rate were evaluated by histological examination of aquaporin 5 (AQP5) and saliva flow rate using the adenylate cyclase (AC) inhibitor SQ22536 (0.25 mg per mouse, s.c.), which was administered 30 min prior to PILO and/or IPR.
- Saliva volume decreased significantly in the mice treated simultaneously with PILO + IPR compared with that in mice treated with PILO alone. Changes in the intracellular localization of AQP5 were seen in PILO + IPR-treated mice, and those changes were reversed by SQ22536 pretreatment. In addition, the decreased salivary flow rate in the PILO + IPR-treated mice was partially restored by SQ22536 pretreatment. There were no significant changes in intracellular calcium or ATP levels among the groups.
- The results of the present study suggest the existence of an inhibitory effect of the sympathetic nervous system on parasympathetic-stimulated salivary secretion from the salivary gland.