These authors contributed equally to this work.
A type VI secretion system regulated by OmpR in Yersinia pseudotuberculosis functions to maintain intracellular pH homeostasis
Article first published online: 24 OCT 2012
© 2012 Society for Applied Microbiology and Blackwell Publishing Ltd
Special Issue: Environmental Ecology of Pathogens and Resistances
Volume 15, Issue 2, pages 557–569, February 2013
How to Cite
Zhang, W., Wang, Y., Song, Y., Wang, T., Xu, S., Peng, Z., Lin, X., Zhang, L. and Shen, X. (2013), A type VI secretion system regulated by OmpR in Yersinia pseudotuberculosis functions to maintain intracellular pH homeostasis. Environmental Microbiology, 15: 557–569. doi: 10.1111/1462-2920.12005
- Issue published online: 28 JAN 2013
- Article first published online: 24 OCT 2012
- Accepted manuscript online: 28 SEP 2012 06:05AM EST
- Manuscript Accepted: 23 SEP 2012
- Manuscript Received: 5 MAY 2012
- National Natural Science Foundation of China. Grant Numbers: 31170100, 31170121, 31270078
- Specialized Research Fund for the Doctoral Program of Higher Education of China. Grant Number: 20110204120023
Type VI secretion systems (T6SSs) which widely distributed in Gram-negative bacteria have been primarily studied in the context of cell interactions with eukaryotic hosts or other bacteria. We have recently identified a thermoregulated T6SS4 in the enteric pathogen Yersinia pseudotuberculosis. Here we report that OmpR directly binds to the promoter of T6SS4 operon and regulates its expression. Further, we observed that the OmpR-regulated T6SS4 is essential for bacterial survival under acidic conditions and that its expression is induced by low pH. Moreover, we showed that T6SS4 plays a role in pumping H+ out of the cell to maintain intracellular pH homeostasis. The acid tolerance phenotype of T6SS4 is dependent on the ATPase activity of ClpV4, one of the components of T6SS4. These results not only uncover a novel strategy utilized by Y. pseudotuberculosis for acid resistance, but also reveal that T6SS, a bacteria secretion system known to be functional in protein transportation has an unexpected function in H+ extrusion under acid conditions.