The bifunctional role of aconitase in Streptomyces viridochromogenes Tü494
Version of Record online: 1 NOV 2012
© 2012 Society for Applied Microbiology and Blackwell Publishing Ltd
Volume 14, Issue 12, pages 3203–3219, December 2012
How to Cite
Michta, E., Schad, K., Blin, K., Ort-Winklbauer, R., Röttig, M., Kohlbacher, O., Wohlleben, W., Schinko, E. and Mast, Y. (2012), The bifunctional role of aconitase in Streptomyces viridochromogenes Tü494. Environmental Microbiology, 14: 3203–3219. doi: 10.1111/1462-2920.12006
- Issue online: 4 DEC 2012
- Version of Record online: 1 NOV 2012
- Accepted manuscript online: 4 OCT 2012 08:33AM EST
- Manuscript Accepted: 24 SEP 2012
- Manuscript Received: 26 JUL 2012
- Deutsche Forschungsgemeinschaft. Grant Number: SPP1152: Wo 485/7-1,2,3
- BMBF. Grant Number: GenBioCom #0315585A
Fig. S1. Phenotypic comparison of S. viridochromogenes WT grown for 6 days in YM medium with different concentrations (2 mM, 10 mM, 20 mM) of (A) tri-sodium citrate, (B) sodium acetate, and (C) succinic acid, disodium salt.
Fig. S2. Analysis of PTT production of the S. viridochromogenes WT, the MacnA mutant, the hisacnA complemented MacnA mutant (MacnA(hisacnA)), the hisacnA1(C538A) complemented MacnA mutant (MacnA(hisacnA1)), and the hisacnA2(Δ125–129) complemented MacnA mutant (MacnA(hisacnA2)) in a biological assay against B. subtilis.
Fig. S3. Transcriptional analysis of recA in the S. viridochromogenes WT and the MacnA mutant (two upper rows). hrdB was used as negative control (two lower rows). The wild-type sample was collected at 48 h and the MacnA sample at 96 h, when both strains were in a comparable growth status. First line: samples treated with the oxygen stress inducer methyl viologen; second line: samples treated with heat stress; third line: samples without stress treatment; fourth line: control – genomic DNA.
Table S1. Primer sequences, amplified fragments, PCR conditions, and IRE sequences.
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