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emi12006-sup-0001-fs1.tif7122K

Fig. S1. Phenotypic comparison of S. viridochromogenes WT grown for 6 days in YM medium with different concentrations (2 mM, 10 mM, 20 mM) of (A) tri-sodium citrate, (B) sodium acetate, and (C) succinic acid, disodium salt.

emi12006-sup-0002-fS2.tif2106K

Fig. S2. Analysis of PTT production of the S. viridochromogenes WT, the MacnA mutant, the hisacnA complemented MacnA mutant (MacnA(hisacnA)), the hisacnA1(C538A) complemented MacnA mutant (MacnA(hisacnA1)), and the hisacnA2(Δ125–129) complemented MacnA mutant (MacnA(hisacnA2)) in a biological assay against B. subtilis.

emi12006-sup-0003-fs3.tif51K

Fig. S3. Transcriptional analysis of recA in the S. viridochromogenes WT and the MacnA mutant (two upper rows). hrdB was used as negative control (two lower rows). The wild-type sample was collected at 48 h and the MacnA sample at 96 h, when both strains were in a comparable growth status. First line: samples treated with the oxygen stress inducer methyl viologen; second line: samples treated with heat stress; third line: samples without stress treatment; fourth line: control – genomic DNA.

emi12006-sup-0004-ts1.doc65K

Table S1. Primer sequences, amplified fragments, PCR conditions, and IRE sequences.

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