Thermal stress responses in the bacterial biosphere of the Great Barrier Reef sponge, Rhopaloeides odorabile
Version of Record online: 26 OCT 2012
© 2012 Society for Applied Microbiology and Blackwell Publishing Ltd
Volume 14, Issue 12, pages 3232–3246, December 2012
How to Cite
Simister, R., Taylor, M. W., Tsai, P., Fan, L., Bruxner, T. J., Crowe, M. L. and Webster, N. (2012), Thermal stress responses in the bacterial biosphere of the Great Barrier Reef sponge, Rhopaloeides odorabile. Environmental Microbiology, 14: 3232–3246. doi: 10.1111/1462-2920.12010
- Issue online: 4 DEC 2012
- Version of Record online: 26 OCT 2012
- Accepted manuscript online: 9 OCT 2012 03:56AM EST
- Manuscript Accepted: 27 SEP 2012
- Manuscript Revised: 24 SEP 2012
- Manuscript Received: 19 JUL 2012
- University of Auckland Doctoral Scholarship
- University of Auckland faculty Research Development Fund. Grant Numbers: 3609286, 3622989
Table S1. blast search results of the top 50 OTUs with the largest negative or positive fold changes represented in Fig. 4A–C. The best match to each OTU is listed along with the accession number, description of the best match and the maximum identity value.
Table S2. Overview of the total reads obtained per sample and the total numbers of OTUs obtained per sample are shown. For the total number of reads, both the raw read numbers and read numbers after noise removal and quality filtering are shown.
Fig. S1. Bacterial diversity in (a) sponge DNA samples, (b) sponge RNA samples, (c) seawater samples. Rarefaction curves are based on OTUs at 97% sequence similarity. Chao1 richness estimates and observed OTUs are listed in parenthesis. Calculations were performed in Mothur (Schloss et al., 2009).
Fig. S2. (a) The proportion of reads that were assigned to an SC or SCC per sample, (b) the proportion of reads that were assigned to an SC per bacterial phylum, and (c) the proportion of reads that were assigned to an SCC per bacterial phylum. The number of reads per phylum (b and c) is calculated as a percentage of the total reads that were assigned to an SC/SCC in each sample. Samples are grouped according to seawater temperature exposure (27°C, 31°C or 32°C) in order of sampling date (days 0, 1, 2, 7, 14 and 21). Sponge samples are grouped separately to seawater samples. Community variation is represented by sponge-derived DNA-based tags (D) versus their activity represented by RNA-derived tags (R).
Fig. S3. Novel and low abundant phylotypes in the phylum Proteobacteria from partially (D332B) and fully (D332C) necrotic sponge clones. Proteobacteria groups were: Vibrionaceae, Pseudoalteromonas, Colwelliaceae, Ferrimonas, Oceanospirillaceae and Arcobacter. These phylotypes were identified from a metagenomic study on thermally stress R. odorabile (L. Fan, pers. obs.). Bar graphs show the number of reads in identified OTUs (a) in all DNA, RNA derived sponge samples and DNA derives seawater samples and (b) in sponge clones exposed to 32°C.
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