Carbohydrate utilization by enterohaemorrhagic Escherichia coli O157:H7 in bovine intestinal content
Version of Record online: 6 NOV 2012
© 2012 Society for Applied Microbiology and Blackwell Publishing Ltd
Special Issue: Environmental Ecology of Pathogens and Resistances
Volume 15, Issue 2, pages 610–622, February 2013
How to Cite
Bertin, Y., Chaucheyras-Durand, F., Robbe-Masselot, C., Durand, A., de la Foye, A., Harel, J., Cohen, P. S., Conway, T., Forano, E. and Martin, C. (2013), Carbohydrate utilization by enterohaemorrhagic Escherichia coli O157:H7 in bovine intestinal content. Environmental Microbiology, 15: 610–622. doi: 10.1111/1462-2920.12019
- Issue online: 28 JAN 2013
- Version of Record online: 6 NOV 2012
- Accepted manuscript online: 10 OCT 2012 07:10AM EST
- Manuscript Accepted: 2 OCT 2012
- Manuscript Revised: 1 OCT 2012
- Manuscript Received: 16 JUL 2012
- EU project (ProSafeBeef) within the 6th Framework Programme. Grant Number: Food-CT-2006–32241
Fig S1. Growth curves of EDL933 incubated in BSIC samples (open circle) and M9-Glc (filled circle). EDL933 was grown at 39°C without shaking. The arrows indicate the time at which the RNA samples were collected. Values are the mean ± 1 SEM of three independent experiments.
Fig. S2. Relative expression of genes required for the catabolism of mucus-derived carbohydrates during incubation of the commensal E. coli strain BG1 in BSIC compared with M9-Glc. The ratio of mRNA level of each gene was measured in BG1 incubated in filtered BSIC in comparison with cells grown in M9-Glc. RNA samples were collected during the exponential growth phase (grey), when the bacteria entered into the stationary phase (white) and during the stationary phase (black). Values are the mean ± 1 SEM of three independent experiments.
Fig. S3. Growth competition assays between the commensal E. coli BG1 and the EHEC strain EDL933 or isogenic mutants of EDL933. The BSIC-LEM samples were inoculated with a 1:1 mixture of the two strains. The EDL933 mutants were defective for the pathway required for the catabolism of fucose (EDL933ΔfucAO), galactose (EDL933ΔgalK), GlcNAc (EDL933ΔnagE), mannose (EDL933ΔmanA) or Neu5Ac (EDL933ΔnanAT). Bars represent the SEM of three independent experiments. The double asterisk denotes statistical significance, P < 0.05 and the triple asterisk denotes statistical significance, P < 0.01 as determined by Student's t-test for paired samples.
Table S1. Genes involved in catabolism of mucus-derived carbohydrates by the EHEC strain EDL933.
Table S2. Bacterial strains and plasmids used in this study.
Table S3. Primer pairs used for gene cloning. The DNA sequence of restriction enzyme site is underlined. Start and stop codons of the cloned genes are in bold.
Table S4. Sequence of primers used in relative mRNA quantification.
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